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    Differences Between RPM and RCF in Centrifugation In centrifuge operation, it is crucial to differentiate between two distinct units of measurement: Relative Centrifugal Force (RCF) and Revolutions Per Minute (RPM). These units are fundamentally different and confusing them—though not uncommon—can compromise the integrity of an experiment. This discussion will explore RCF and RPM in greater detail and examine their relationship. **Revolutions Per Minute (RPM) Revolutions Per Minute (RPM) refers to the measurement of the rotational speed of a centrifuge rotor, indicating the number of complete rotations it performs in one minute. This metric quantifies the rotor's spinning velocity. Each centrifuge has a specific speed range that varies by model. For instance, a low-speed centrifuge may operate at a minimum of 300 RPM, while a high-speed centrifuge can reach speeds of up to 15,000 RPM. Additionally, ultracentrifuges, the most powerful type, can exceed 150,000 RPM. **Relativ...